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Prep asparagus: wash, pat dry, and using kitchen shears cut off about 1 to 2 inches off the bottom so the woody end of the asparagus is removed

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The variable weight value (VIP) in Orthogonal Partial Least Squares-Discriminant Analysis is a measure of the expression pattern of each metabolite, the explanatory ability and depth of influence on the categorical discrimination of samples in each group, to explore meaningful differential metabolites, and then use the T-test to verify whether the differential metabolites between groups are significant