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The study looked for both morphological changes as well as behavioral changes and found methylene blue to significantly alleviate brain injury both if administered immediately after brain injury and if administered 24 hours after the injury.8 Another randomized, double-blinded, vehicle-controlled trial evaluated oral methylene blue 4mg/kg for 21 days concluded that long term oral administration of low dose methylene blue reduced brain lesion volume and white matter damage after stroke induction in rats

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