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VEGF signaling inhibits BMP pathway activity to block trophoblast induction To elucidate how VEGF signaling sustained hESC self-renewal and repressed trophoblast lineage specification, genome-wide transcriptional profiles were analyzed by RNA-sequencing (RNA-seq) on hESCs treated with a VEGFR inhibitor or OE of soluble VEGF receptors at multiple time points: Len or Tiv for 3 h, 6 h, 12 h, and 18 h
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