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Anatrace, D310), 50LmM HEPES (pH-adjusted with KOH, pHL7.5), 150LmM KCl supplemented with protease-inhibitor cocktail (1LmM PMSF, 2.5LmgLml −1 aprotinin, 2.5LmgLml −1 leupeptin, 1LmgLml −1 pepstatin A) and DNase I