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Estimation of total starch and resistant starch The collected samples (0.1 g) were fractionated in 80% ethanol (hot) as per the protocol of RS in the grain was estimated using the Resistant Starch Assay kit (Megazyme) ( Estimation of total phenolic content The total phenolic content was estimated using the method of Estimation of total tannin content The tannin content was estimated using the Folin–Ciocalteu reagent method ( 2 CO 3 ), and 7.5 mL of double-distilled water, followed by incubation at RT for 30 min