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Signals were detected using horseradish peroxidase-conjugated secondary antibodies and ECL Prime Western Blotting Detection Reagent (GE Healthcare) on a Doc™ XRS + (Bio-Rad)
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Knockdown of TMEM19 by siRNA results in failure to respond to the erucamide-stimulated paracrine effect of growth factors, which is consistent with in vivo knockdown of TMEM19 and inhibition of erucamide–TMEM19 downstream pathways by neutralizing antibodies (Fig
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