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The study protocol was approved by the medical ethics committee of the Maastricht University Medical Center+, the Netherlands and was in line with the Helsinki Declaration of Human Rights (identifier: NL73600.068.20/METC 20–033)

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Previous studies have identified that circadian proteins could be responsible for aging through the regulation of metabolism, genotoxic stress response and reactive oxygen species (ROS) homeostasis ( Role of BMAL1 in Oxidative Stress Oxidative stress refers to an imbalance between oxidants and antioxidants in favor of oxidants, which leads to a disruption of redox signaling and molecular damage ( A major characteristic of aging is an increase in oxidative stress, which is considered one of the molecular mechanisms underlying the occurrence and development of a variety of age-related pathologies