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Specifically, 20 μL of pFeSAN (1 mg/mL) and 20 μL of TMB (50 mM) were added into HAc-NaAc buffer (0.1 M, pH 4.0), which contains 0.1 mM GSH, Na + , Ca 2+ , Mg 2+ , K + , Glu, Trp, Arg, Gly, Cys, L-AA, glucose, GOx or BSA