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Study 1–10 co-injections: Groups of rats (n = 9 per group) received co-injections (a) vehicle (100 μL/100g body weight, IV) + fentanyl (125 μg/kg, IV), (b) L-NAC (500 μmol/kg, IV) + fentanyl (125 μg/kg, IV), or (c) L-NACme (500 μmol/kg, IV) + fentanyl (125 μg/kg, IV) given 90 s apart at 8 AM and 8 PM on days 1 to 4

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Immunoreactive bands on the membrane were visualized with ECL select (GE Healthcare) and scanned with a LAS-4000mini LuminoImage analyzer (Fuji Film, Tokyo, Japan)

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