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STZ-treated mice exhibited a markedly decreased insulin-positive area and a severe reduction in the β-cell proportion in the islets, with an expansion of α-cells infiltrating into the center of the islets (Figures 3A–D)

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% in water, Sigma-Aldrich, #F8775) and HCl (0.5 mL, 36.5–38.0 %) were dissolved in 2 mL of dioxane (Sintorgan, #SIN-083003-63)