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Neelakantan and colleagues (Biochem Pharmacol, 2017) then took the pharmacological step, reporting that selective, membrane-permeable small-molecule NNMT inhibitors reversed high-fat-diet-induced obesity in mice — this is the study most directly relevant to the 5-Amino-1MQ class, because it moves from “the target matters” to “a drug against the target does something in a live animal.” And again, it is a mouse study