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Weeks 3-4: Peak Cycle Benefits For protocols that extend to 3-4 weeks, this period represents peak accumulated benefit

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Cells were stained for 30 min on ice, washed twice in FACS buffer, fixed in 200 µl of 10% formalin 15 min on ice, washed and resuspended in 200 µl FACS buffer for acquisition and analyzed on a BDFACS Celesta