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Nach der Abgabe eines Elektrons oxidiert Glutathion zu GSSG (oxidiertes Glutathion) - die Glutathionreduktase, die NADPH benötigt, regeneriert es zurück in die aktive Form GSH

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Subsequently, the immunoprecipitates were further washed with kinase buffer containing 20 mM MgCl₂, 100 µM ATP, 2 mM DTT, and 50 µM of specified inhibitors, in addition to 40 mM Tris-HCl (pH 7.5)