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2) NLRP3 inflammasome activation, triggered by Tat, gp120, and viral RNAs, which is exacerbated by ROS, ion flux changes, and impaired autophagy, with NF-κB acting as a crucial priming signal for pyroptosis and pro-inflammatory cytokine release

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The virus supernatant was used to transduce bone marrow-derived macrophages (BMMs), including M-CSF and 20 g/ml of protamine (Sigma-Aldrich)