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The beads were washed three times with NETN buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% NP-40), twice with ETN buffer (50 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1 mM EDTA) and once with purified water to remove impurities

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Choosing Based on Skin Goals For Elasticity and Firmness: Choose Vitamin C to support collagen frameworks