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Cancer (Preclinical) Silymarin and its constituent silibinin have demonstrated anti-cancer properties in laboratory and animal studies [3][45][46]

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Cell Cultures OLN-93 cells, a cell line established from spontaneously transformed rat brain glial cultures [46], were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA), penicillin, and streptomycin at 37 °C

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