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Modern testing (expanded quantitative urine culture and 16S ribosomal RNA gene sequencing) can detect previously unrecognised organisms, making it possible to answer previously intractable scientific and clinical questions
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45–47 In mouse epithelia, ZO-1 depletion was shown to cause delayed TJ formation and reassembly with integrity being unaffected, but TJ disruption was not detected in ZO-2-depleted cells
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