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The key comparison was between the KC group (KD with biotin) and the KBD group (KD without biotin), which allowed the researchers to assess whether KD alone increases biotin requirements or accelerates the development of biotin deficiency

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Approximation of the slopes and intercept allowed estimation of apparent K m and V max values from Lineweaver-Burk and Hofstee linear plots were: 27.62 μM and 0.66 nmoles ml -1 min -1

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