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Transfection, BSO treatment, and exposure of AQ-QI and DEAQ-QI were identical as described in section “Transient Transfection of Human GSTP1 Gene” and “Cell Viability Assay.” After exposure the medium was gently removed and the later steps were conducted according to manufacturer’s manual
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As evidenced, at maximal glucose stimulation, the rising of NAD(P)H levels is estimated to be approximately 30 mM in whole pancreatic islet beta cells, with a 7 mM in the cytoplasmic domain and an approximately 20 second delayed 60 mM in mitochondrial domain
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