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These findings indicate that the action of endogenous leptin is intact in mice with DIO despite hyperleptinaemia and resistance to exogenous leptin, thus challenging the view that leptin no longer suppresses food intake and reduces body weight in obesity

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Preparation of cell extract for evaluation of enzyme activities Controls and patient fibroblasts (6 × 10 6 cells/ml) were lysed in 100 mM phosphate buffer (pH 7), containing 1.5 mM dithiotreitol (DTT) and 1 mM EDTA for total glutathione peroxidase and 100mM phosphate buffer (pH 7), containing 0.1% (v/v) Triton X-100 for total SOD, SOD2 and CAT