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After TMB substrate solution is added, only those wells that contain Rat GROa/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

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Furthermore, a deficiency of PGC-1α has been identified as a factor that inhibits the activity and function of dopaminergic neurons and causes behavioral dysfunction in the mouse central nervous system (Kuczynska et al., 2021)