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The importance of spleen, spleen iron, and splenectomy for determining total body iron load, ferrikinetics, and iron toxicity in thalassemia major patients

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Downstream TERT activation elongates critically short telomeres (measured by Southern blot TRF analysis, or by qPCR telomere length ratio T/S), delays the p16-p21-Rb senescence programme (SA-β-gal pH 6.0 reduction, p16 Ink4a western blot decrease), and reduces the senescence-associated secretory phenotype (SASP: IL-6, IL-8, MMP-1, MMP-3, GDF15 multiplex Luminex panel in conditioned media from late-passage HDFs)