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The reaction mixture (1 ml) containing Locke’s buffer (pH 7.4), 0.2 ml homogenate and 10 μl of DCFH-DA (5 mM) was incubated for 45 min at room temperature and the fluorescence intensity of DCF was recorded by excitation at 485 nm and emission at 535 nm using a Synergy TM 2 Multi-function Microplate Reader (Bio-Tek Instruments Inc., Winooski, Vermont, USA)
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