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The Central Nervous System Modulatory Activities of N-Acetylcysteine: A Synthesis of Two Decades of Evidence

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Tissues and cell pellets were lysed in RIPA lysis buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) (Pierce, Thermo Fisher Scientific) and protease (cOmplete, Sigma-Aldrich) and phosphatase (phosSTOP, Sigma-Aldrich) inhibitors