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Primary antibodies used were p53 (CST, #9282, 1:1000 dilution), phospho-p53 (CST, #9284, 1:1000 dilution), p21 (CST, #2947, 1:1000 dilution), phospho-histone H2A.X (CST, #9718, 1:1000 dilution), phospho-CHK1 (CST, #2348, 1:500 dilution), phospho-CHK2 (CST, #2661, 1:500 dilution), Vinculin (Santa Cruz, #sc-73614, 1:2000 dilution), alpha-tubulin (CST, #2144, 1:2000 dilution), and beta-actin (Santa Cruz, #sc-47778, 1:2000 dilution)