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Duration:45mins

To compare cell death between FSP1–GFP expression lines (WT, RFK KO , FLAD1 KO and no-Dox control), the SYTOX-object counts at the 24-h time point from 1 µM RSL3 treatment were normalized by the maximum value for each cell line (that is, the phase-object counts at the 0-h time point)

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