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Preparation of Dual hGH Fragment 176–191 Peptide- and Doxorubicin-Loaded Chitosan Nanoparticles (Ch-hGH-DOX) Chitosan nanoparticles were synthesized using the ionic gelation method of Avadi et al,30 with slight modification.31 Negatively charged gum Arabica was dissolved in water at normal room temperature under constant stirring, while positively charged LMW Chitosan was dissolved in 0.1% aqueous acetic acid also at normal room temperature, and under constant stirring
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