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The cell-free supernatant was combined with a sulfhydryl preserving solution and assayed for glutathione content by HPLC and normalized based on the urea dilution method as described previously ( STATISTICS Nominal data are presented as means with SE and analyzed by ANOVA and Dunnet’s or Bennett’s post hoc test as indicated

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In contrast to synthetic agents, these resources are regarded as secure and efficacious alternatives that exhibit a reduced incidence of adverse effects (59)