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Two primary tests measure the amount of B12 in blood (low in B12 deficiency): Total B12 test: measures total amount of B12 Active B12 test: measures amount of B12 that is available to the cells Two secondary tests measure function of B12 in the cell (raised in B12 deficiency): MMA: measures function of B12 in the mitochondria Homocysteine: measures function of B12 for methylation How does Vitamin B12 deficiency get diagnosed