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Following euthanasia by isoflurane, the portal vein was cannulated using a 24G IV catheter (Terumo, SR-OX2419CA)

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Furthermore, certain lipoxygenases (LOXs) have been found to be involved in ferroptosis because of their ability to directly oxygenate PUFAs and PUFA-containing lipids in biological membranes[36, 38, 39] (Figure 2)