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Administration of the ED 80 po antinociceptive dose of mitragynine resulted in detection of 0.094 ± 0.03 and 0.035 ± 0.01 μ M concentrations of 3DM in the brains of C57BL/6J and 129Sv6 mice, respectively, coinciding with concentrations of 0.037 ± 0.01 and 0.028 ± 0.01 μ M in plasma of C57BL/6J and 129Sv6 mice, respectively (Figure 7D)
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