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Muscles were digested with 8 mg ml −1 collagenase D (Roche) and 10 U ml −1 Dispase II (Roche) and then manually dissociated to generate cell suspensions

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While the majority of published data originates from the Sikiric laboratory in Zagreb, independent groups have increasingly contributed to the literature, and the peptide remains a subject of active investigation across multiple areas of preclinical science.