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The thiol moiety present in glutathione exhibits a reduction reaction with Ellman's reagent, also known as 5,5-dithio-bis-2-nitrobenzoic acid leading to a yellow-colored compound (5-thio-2-nitrobenzoic acid), which was quantified spectrophotometrically at 412 nm 55
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The combination of these two interactions tether the latent TGFβ complex at both ends, such that cellular contractile forces release the mature TGFβ ligand from the prodomain 42, 45, 46
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