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After treating human hepatocytes with prednisolone, the active metabolite of prednisone, and CsA at concentrations ranging from 0.5 to 100 μM for 96 h, the incubation medium was replaced with 100 μM 4-trifluoromethyl-7-hydroxycoumarin and 100 μM β-estradiol, which are probe substrates of UGT1A9/2B7 and UGT1A1/1A3, respectively, for an additional incubation of 30 min
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