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For orthotopic liver tumor models, the Hepa1–6 cells (5 × 10 6 ) were suspended in 25 μL of Matrigel (Sigma-Aldrich) and inoculated into the liver parenchyma of the left lobe for in situ liver tumor model ( n = 6)
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Program:8 Week Skin and Tissue Optimization
Any vial showing visible contamination must be discarded immediately
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