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Even a small mistake in positioning or pace can send liquid the wrong way, especially if the goat is startled or struggling
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This would result if the bioavailable pool of a particular metal is insufficient to metalate all cognate metalloproteome sites quantitatively, and is of course a prediction of the set-point metallostasis model, where the free energy of (mis)metalation is positive (+ΔΔ G ) (Figure 8B)
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