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Methods Sample preparation for ice nucleation experiments Aqueous solutions were prepared at concentrations of 5 mg mL −1 , 0.5 mg mL −1 , and 5 × 10 −4 mg mL −1 using ultrapure water (HPLC water, Sigma-Aldrich) and commercially available compounds, which were obtained from Sigma-Aldrich unless otherwise noted

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Studies on the involvement of individual DC subpopulations in the development of inflammatory infiltrates in morphea are still outstanding